Effects of Preprocessing of Ultraviolet-Induced Fluorescence Spectra in Plant Fingerprinting Applications

$29.00 plus tax (Refund Policy)

Buy Article:


Preprocessing is an important step in data analysis. Dealing with spectral data, normalization is mandatory in order to compare items collected under various conditions. This paper addresses normalization of front-face fluorescence spectroscopy data where spectra are affected by an unknown multiplicative effect. The usual methods for reducing multiplicative problems are reviewed and a more detailed analysis of the normalization by closure is provided based on data on the fluorescence of plants as a means for plant species fingerprinting. As normalization is essentially the reduction of information, some methods of carrying it out are likely to remove either meaningful or discriminant pieces of information. As a result, it is demonstrated that normalization by closure should be performed using spectral data in a range where the spectra contain no information relevant to the problem at hand. This applies provided that in this range the signal-to-noise ratio is high enough. When the noise level is too high, a compromise should be found between preserving useful information and limiting the amount of noise introduced by the normalization procedure. Even if this study were carried out using fluorescence spectra, the overall process is likely to be applied to other spectral data.


Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370208784909661

Affiliations: 1: Horticultural R&D Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, QC, Canada, J3B 3E6 2: Cemagref, Montpellier, France 3: Laval University, Quebec, Canada

Publication date: July 1, 2008

More about this publication?
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more