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A Multi-Source Portable Light Emitting Diode Spectrofluorometer

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Abstract:

A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (<1.5 kg) spectrometer. Limits of detection for rhodamine 6G, rhodamine B, and fluorescein were 2.9, 3.2, and 11.0 nM, respectively. Generation of excitation-emission matrices (EEMs) enabled the analysis of samples containing mixtures of rhodamine B and fluorescein. Buffered saline plant and animal feed extracts were also analyzed using this instrument. These samples included the woody plants Pistacia lentiscus (Evergreen pistache or Mastic) and Philyria latifolia, and the herbaceous species Medicago sativa (alfalfa), Trifolium spp. (clover), and a feed concentrate. Application of multi-way principal component analysis (MPCA) to the resulting three-dimensional data sets enabled discernment among these various diet constituents.

Keywords: ANIMAL FORAGE; EEM; EXCITATION-EMISSION MATRICES; FLUORESCENCE; LEDS; LIGHT EMITTING DIODES; PORTABLE SPECTROMETER

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370208783759722

Affiliations: 1: Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003-0001 2: Box 30001 MSC JER, United States Department of Agriculture, Agriculture Research Service, Jornada Experimental Range, Las Cruces, New Mexico 88003-0003 3: Department of Natural Resources and Agronomy, Institute of Plant Science, Agriculture Research Organization, The Volcani Centre, Bet Dagan, Israel 50250

Publication date: March 1, 2008

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