Construction of a Subnanosecond Time-Resolved, High-Resolution Ultraviolet Resonance Raman Measurement System and Its Application to Reveal the Dynamic Structures of Proteins

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

A subnanosecond time-resolved ultraviolet (UV) resonance Raman system has been developed to study protein structural dynamics. The system is based on a 1 kHz Nd:YLF-pumped Ti:Sapphire regenerative amplifier with harmonic generation that can deliver visible (412, 440, 458, and 488 nm) and UV (206, 220, 229, and 244 nm) pulses. A subnanosecond (0.2 ns) tunable near-infrared pulse from a custom-made Ti:Sapphire oscillator is used to seed the regenerative amplifier. A narrow linewidth of the subnanosecond pulse offers the advantage of high resolution of UV resonance Raman spectra, which is critical to obtain site-specific information on protein structures. By combination with a 1 m single spectrograph equipped with a 3600 grooves/mm holographic grating and a custom-made prism prefilter, the present system achieves excellent spectral (<10 cm−1) and frequency (∼1 cm−1) resolutions with a relatively high temporal resolution (<0.5 ns). We also report the application of this system to two heme proteins, hemoglobin A and CooA, with the 440 nm pump and 220 nm probe wavelengths. For hemoglobin A, a structural change during the transition to the earliest intermediate upon CO photodissociation is successfully observed, specifically, nanosecond cleavage of the A–E interhelical hydrogen bonds within each subunit at Trpα14 and Trpβ15 residues. For CooA, on the other hand, rapid structural distortion (<0.5 ns) by CO photodissociation and nanosecond structural relaxation following CO geminate recombination are observed through the Raman bands of Phe and Trp residues located near the heme. These results demonstrate the high potential of this instrument to detect local protein motions subsequent to photoreactions in their active sites.

Keywords: COOA; HEMOGLOBIN; INSTRUMENTATION; TIME-RESOLVED ULTRAVIOLET RESONANCE RAMAN SPECTROSCOPY; UVRR SPECTROSCOPY; VIBRATIONAL SPECTROSCOPY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370208783412573

Affiliations: 1: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Department of Physics, Northeastern University, Boston, MA 02115 2: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan 3: Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, Toyonaka 560-8531, Japan 4: Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan; Toyota Physical & Chemical Research Institute, Aichi 480-1192, Japan

Publication date: January 1, 2008

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more