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Raman Spectroscopic Measurement of Relative Concentrations in Mixtures of Oral Bacteria

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Abstract:

Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens for more than a decade. More recently, this optical method has been extended to the analysis of specimens containing multiple species. In this report, we demonstrate rapid, reagent-free quantitative analysis of a simplified model of oral plaque containing three oral bacteria species, S. mutans, S. sanguis, and S. gordonii, using near-infrared Raman spectroscopy. Raman spectra were acquired from bacterial mixtures in 200 seconds. A prediction model was calibrated by the partial least squares method and validated by additional samples. On a scale from 0 to 1, relative fractions of each species could be predicted with a root mean square error of 0.07. These results suggest that near-infrared Raman spectroscopy is potentially useful in quantification of microbial mixtures in general and oral plaques in particular.

Keywords: BACTERIA; MICROORGANISMS; ORAL; PARTIAL LEAST SQUARES; PLS; QUANTIFICATION; RAMAN SPECTROSCOPY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370207782597021

Affiliations: 1: The Institute of Optics, University of Rochester, Rochester, New York 14627 2: Center for Oral Biology, Aab Institute for Biomedical Sciences, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642

Publication date: November 1, 2007

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