Authors: Zhao, Jianhua; Lui, Harvey; McLean, David I.; Zeng, Haishan

Source: Applied Spectroscopy, Volume 61, Issue 11, Pages 248A-270A and 1135-1264 (November 2007) , pp. 1225-1232(8)

Publisher: Society for Applied Spectroscopy

Abstract:

A significant advantage of Raman spectroscopy as a noninvasive optical technique is its ability to detect subtle molecular or biochemical signatures within tissue. One of the major challenges for biomedical Raman spectroscopy is the removal of intrinsic autofluorescence background signals, which are usually a few orders of magnitude stronger than those arising from Raman scattering. A number of methods have been proposed for fluorescence background removal including excitation wavelength shifting, Fourier transformation, time gating, and simple or modified polynomial fitting. The single polynomial and the modified multi-polynomial fitting methods are relatively simple and effective, and thus are widely used in biological applications. However, their performance in real-time in vivo applications and low signal-to-noise ratio environments is sub-optimal. An improved automated algorithm for fluorescence removal has been developed based on modified multi-polynomial fitting, but with the addition of (1) a peak-removal procedure during the first iteration, and (2) a statistical method to account for signal noise effects. Experimental results demonstrate that this approach improves the automated rejection of the fluorescence background during real-time Raman spectroscopy and for in vivo measurements characterized by low signal-to-noise ratios.

Keywords: RAMAN SPECTROSCOPY; FLUORESCENCE BACKGROUND REMOVAL; BIOMEDICAL RAMAN; POLYNOMIAL FITTING

Document Type: Research article

DOI: http://dx.doi.org/10.1366/000370207782597003

Affiliations: 1: The Laboratory for Advanced Medical Photonics (LAMP), Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute and Cancer Imaging Department, BC Cancer Research Center, Vancouver,

Publication date: 2007-11-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Zhao, Jianhua ;  Lui, Harvey ;  McLean, David I. ;  Zeng, Haishan

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers