Authors: Liu, Yongliang¹; Chen, Yud-Ren²; Nou, Xiangwu³; Chao, Kuanglin²

Source: Applied Spectroscopy, Volume 61, Issue 8, Pages 150A-170A and 797-907 (August 2007) , pp. 824-831(8)

Publisher: Society for Applied Spectroscopy

Abstract:

Surface-enhanced Raman (SERS) spectra of various batches of bacteria adsorbed on silver colloidal nanoparticles were collected to explore the potential of the SERS technique for rapid and routine identification of E. coli and L. monocytogenes cultures. Relative standard deviation (RSD) of SERS spectra from silver colloidal suspensions and ratios of SERS peaks from small molecules (K₃PO₄) were used to evaluate the reproducibility, stability, and binding effectiveness of citrate-reduced silver colloids over batch and storage processes. The results suggested consistent reproducibility of silver colloids over batch process and also stability and consistent binding effectiveness over an eight-week storage period. A variety of mixtures of E. coli/L. monocytogenes cultures with different colloidal batches revealed that, despite large variations in relative intensities and positions of SERS active bands, characteristic and unique bands at 712 and 390 cm⁻¹ were consistently observed and were the strongest in E. coli and L. monocytogenes cultures, respectively. Two specific bands were used to develop simple algorithms in the evaluation of binding effectiveness of silver colloids over storage and further to identify E. coli and L. monocytogenes cultures with a 100% success. A single spectrum acquisition took 5∼6 min, and a minimum of 25 μL silver colloid was directly mixed with 25 μL volume of incubated bacterial culture. The short acquisition time and small volume of incubated bacterial culture make silver colloidal nanoparticle based SERS spectroscopy ideal for potential use in the routine and rapid screening of E. coli and L. monocytogenes cultures on large scales. This is the first report of the development of simple and universal algorithms for bacterial identification from the respective exclusive SERS peaks.

Keywords: E. COLI BACTERIA; L. MONOCYTOGENES BACTERIA; SURFACE-ENHANCED RAMAN SPECTROSCOPY; SERS; SILVER COLLOID NANOPARTICLES; ALGORITHM; BACTERIAL IDENTIFICATION; SAFETY

Document Type: Research article

DOI: http://dx.doi.org/10.1366/000370207781540060

Affiliations: 1: Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742 2: Instrumentation and Sensing Laboratory, Henry A.Wallace Beltsville Agricultural Research Center, USDA, Building 303, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705 3: Food Technology and Safety Laboratory, Henry A.Wallace Beltsville Agricultural Research Center, USDA, Building 201, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705

Publication date: 2007-08-01

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