Standard Reference Materials® SRMs 2241 through 2243 are certified spectroscopic standards intended for the correction of the relative intensity of Raman spectra obtained with instruments employing laser excitation wavelengths of 785 nm, 532 nm, or 488 nm/514.5 nm. These
SRMs each consist of an optical glass that emits a broadband luminescence spectrum when illuminated with the Raman excitation laser. The shape of the luminescence spectrum is described by a polynomial expression that relates the relative spectral intensity to the Raman shift with units in
wavenumber (cm−1). This polynomial, together with a measurement of the luminescence spectrum of the standard, can be used to determine the spectral intensity-response correction, which is unique to each Raman system. The resulting instrument intensity-response correction may
then be used to obtain Raman spectra that are corrected for a number of, but not all, instrument-dependent artifacts. Peak area ratios of the intensity-corrected Raman spectrum of cyclohexane are presented as an example of a methodology to validate the spectral intensity calibration process
and to illustrate variations that can occur in this measurement.
Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8394
Publication date: February 1, 2007
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