Measurement of Contrast Transfer Function in Super-Resolution Microscopy Using Two-Color Fluorescence Dip Spectroscopy

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The contrast transfer function (CTF) of super-resolution microscopy was quantitatively investigated using a fluorescent scale. The scale has minute fluorescent line patterns, finer than 100 nm, and is suitable for measuring CTF in super-resolution microscopy. The measured CTF shows that super-resolution microscopy can indeed improve the optical properties of fluorescent images and enable us to observe a structure with the spatial resolution overcoming the diffraction limit. From the CTF, it has been found that super-resolution microcopy can resolve a 100 nm line-and-space pattern and provides a contrast of 10%. The CTF corresponds to a PSF with a full-width at half-maximum (FWHM) of 130 nm. An evaluation using a 100 nm fluorescent bead consistently supports the results given by the CTF for super-resolution microscopy.

Keywords: CONTRAST TRANSFER FUNCTION; FLUORESCENCE-DEPLETION PROCESS; FLUORESCENT SCALE; RHODAMINE 6G; SUPER-RESOLUTION MICROSCOPY; TWO-COLOR DIP SPECTROSCOPY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370207779701523

Affiliations: 1: Olympus Corporation, 2–3 Kuboyama-cho, Hachioji, 192-8512, Japan 2: Budapest University of Technology and Economics, Dept. Physics, Budapest 1111, Hungary 3: Chiba Univ. Dept. Information & Imaging Science, 1–33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan 4: National Institute of Advanced Industrial Science & Technology, Photonics Research Institute, 1-8-31 Midorigaoka, Ikeda, 563-8577, Japan 5: Keio Univ. Dept. of Chemistry, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan 6: Tokyo Institute of Technology, Chemical Resources Laboratory, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan

Publication date: January 1, 2007

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more