Skip to main content

Measurement of Contrast Transfer Function in Super-Resolution Microscopy Using Two-Color Fluorescence Dip Spectroscopy

Buy Article:

$29.00 plus tax (Refund Policy)

Abstract:

The contrast transfer function (CTF) of super-resolution microscopy was quantitatively investigated using a fluorescent scale. The scale has minute fluorescent line patterns, finer than 100 nm, and is suitable for measuring CTF in super-resolution microscopy. The measured CTF shows that super-resolution microscopy can indeed improve the optical properties of fluorescent images and enable us to observe a structure with the spatial resolution overcoming the diffraction limit. From the CTF, it has been found that super-resolution microcopy can resolve a 100 nm line-and-space pattern and provides a contrast of 10%. The CTF corresponds to a PSF with a full-width at half-maximum (FWHM) of 130 nm. An evaluation using a 100 nm fluorescent bead consistently supports the results given by the CTF for super-resolution microscopy.

Keywords: CONTRAST TRANSFER FUNCTION; FLUORESCENCE-DEPLETION PROCESS; FLUORESCENT SCALE; RHODAMINE 6G; SUPER-RESOLUTION MICROSCOPY; TWO-COLOR DIP SPECTROSCOPY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370207779701523

Affiliations: 1: Olympus Corporation, 2–3 Kuboyama-cho, Hachioji, 192-8512, Japan 2: Budapest University of Technology and Economics, Dept. Physics, Budapest 1111, Hungary 3: Chiba Univ. Dept. Information & Imaging Science, 1–33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan 4: National Institute of Advanced Industrial Science & Technology, Photonics Research Institute, 1-8-31 Midorigaoka, Ikeda, 563-8577, Japan 5: Keio Univ. Dept. of Chemistry, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan 6: Tokyo Institute of Technology, Chemical Resources Laboratory, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Japan

Publication date: January 1, 2007

More about this publication?
sas/sas/2007/00000061/00000001/art00007
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more