Skip to main content

Comparison of Methods for Reducing the Effects of Scattering in Spectrophotometry

Buy Article:

$29.00 plus tax (Refund Policy)

Abstract:

Light scattering provides a problem in optical spectroscopy as the relationship between attenuation and absorption in the presence of scattering is nonlinear. Three simple methods of reducing the effects of scattering and hence returning to an approximately linear relationship are considered in this paper, namely, extracting light that has maintained its original polarization state through subtraction of orthogonal polarization states, use of an added absorber, and spatial filtering. These can all be applied relatively easily to conventional spectrophotometers. However, there is an inevitable trade-off between the accuracy of the measurement and the signal-to-noise ratio as scattered light is rejected from the detector. It is demonstrated that polarization subtraction is the most efficient technique at selecting weakly scattered photons from a scattered light background as it enables the relationship between attenuation and absorption coefficient to become more linear while maintaining a higher number of detected photons. In practical use, the drawback of polarization subtraction over added absorber and spatial filtering methods is that a large dc background light level is maintained, which contributes to a higher shot noise. This means that when the scattering coefficient is high (μs ≥ 7 mm-1) the added absorber method offers better performance for shot noise limited detection.

Keywords: ABSORPTION; ADDED ABSORBER; ATTENUATION; LAMBERT-BEER LAW; POLARIZATION SUBTRACTION; SCATTERING; SPATIAL FILTERING

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370206778664725

Affiliations: School of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham, United Kingdom

Publication date: October 1, 2006

More about this publication?
sas/sas/2006/00000060/00000010/art00014
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more