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Infrared Transmission Spectrometry for the Determination of Urea in Microliter Sample Volumes of Blood Plasma Dialysates

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Abstract:

Application of mid-infrared spectroscopy for the determination of urea in blood plasma dialysates of microliter sample volumes using a transmission microcell was investigated. Infrared spectra of the dialysates of plasma samples collected from 75 different patients using CMA 60 microdialysis catheters were evaluated with multivariate partial least squares regression. Using the absorbance spectral data from 1520–1420 cm−1 and 1220–1120 cm−1, a minimum standard error of prediction (SEP) of 0.88 mg/dL (0.14 mM) was achieved with spectral variable selection. Our findings suggest the feasibility of developing a mid-infrared sensor in combination with micro-fluidics for on-line monitoring of urea in patients undergoing dialysis treatment.

Keywords: DIALYSIS; MID-INFRARED SPECTROSCOPY; PARTIAL LEAST SQUARES REGRESSION; PLSR; SPECTRAL VARIABLE SELECTION; UREA

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370206778062066

Affiliations: ISAS—Institute for Analytical Sciences at the University of Dortmund, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, Germany

Publication date: August 1, 2006

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