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Resonance Raman Evidence of Immobilization of Laccase on Self-Assembled Monolayers of Thiols on Ag and Au Surfaces

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Abstract:

Resonance Raman (RR) and surface-enhanced Raman scattering (SERS) spectroscopy have been used to study immobilization of laccase on self-assembled monolayers (SAMs) of thiols containing carboxylic and amino groups, deposited on silver and gold electrodes. A new, indirect way of monitoring laccase bound to the thiol-coated Ag and Au surfaces is presented. It was demonstrated that by recording the resonance Raman spectra of the colored product of the oxidation of syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehydeazine) by laccase in the presence of molecular oxygen, one may easily confirm binding as well as enzymatic activity of laccase immobilized on the SAMs modified silver and gold surfaces.

Keywords: LACCASE IMMOBILIZATION; RESONANCE RAMAN; SERS; SURFACE-ENHANCED RAMAN SCATTERING

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370206777887035

Affiliations: Department of Chemistry, University of Warsaw, Pasteur 1, 02–093 Warszawa, Poland

Publication date: July 1, 2006

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