Temperature-Jump Apparatus with Raman Detection Based on a Solid-State Tunable (1.80–2.05 μm) kHz Optical Parametric Oscillator Laser

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Abstract:

The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (∼10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing ∼1 mJ NIR pulses that are tunable (1.80–2.05 μm) across the 1.9 μm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 °C, as measured via the O–H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated.

Keywords: CYTOCHROME C; NEAR-INFRARED LASER; OPTICAL PARAMETRIC OSCILLATOR; PROTEIN FOLDING; TEMPERATURE JUMP; ULTRAVIOLET RESONANCE RAMAN

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370206776593799

Affiliations: 1: Department of Chemistry, Princeton University, Princeton, New Jersey 08544 2: Department of Chemistry, Princeton University, Princeton, New Jersey 08544; Department of Food Science, Cornell University, Ithaca, NY 14853

Publication date: April 1, 2006

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