Surface-Enhanced Raman Spectroscopy of Chernozem Humic Acid and Their Fractions Obtained by Coupled Size Exclusion Chromatography-Polyacrylamide Gel Electrophoresis (SEC-PAGE)

Authors: Sanchez-Cortes, S.¹; Corrado, G.¹; Trubetskaya, O.E.²; Trubetskoj, O.A.³; Hermosin, B.⁴; Saiz-Jimenez, C.⁴

Source: Applied Spectroscopy, Volume 60, Issue 1, Pages 11A-25A and 1-107 (January 2006) , pp. 48-53(6)

Publisher: Society for Applied Spectroscopy

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Abstract:

A humic acid extracted from a chernozem soil was fractionated combining size exclusion chromatography and polyacrylamide electrophoresis (SEC-PAGE). Three fractions named A, B, and C+D, with different electrophoretic mobilities and molecular sizes (MS), were obtained and subsequently characterized by thermochemolysis and surface-enhanced Raman spectroscopy (SERS). The data confirmed that fraction A, with the higher MS, was more aliphatic than fractions B and C+D and, in turn, fractions with lower MS (B and C+D) denoted an enrichment in lignin residues. These structural features explain conformational changes when varying the pH in the humic fraction A and indicated that combination of the two techniques is a good approach for characterizing humic substances.

Keywords: SURFACE-ENHANCED RAMAN SPECTROSCOPY; SERS; HUMIC ACID; CHERNOZEM SOIL; SIZE EXCLUSION CHROMATOGRAPHY; POLYACRYLAMIDE ELECTROPHORESIS; SEC-PAGE; THERMOCHEMOLYSIS

Document Type: Research article

DOI: 10.1366/000370206775382695

Affiliations: 1: Instituto de Estructura de la Materia, Consejo Superior de Investigaciones Cientificas, Serrano 121, 28006 Madrid, Spain 2: Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Moscow region, Russia 3: Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow region, Russia 4: Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Apartado 1052, 41080 Sevilla, Spain

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