Calcium Fluoride Micro Cells for Synchrotron Radiation Circular Dichroism Spectroscopy

Authors: Wien, F.1; Wallace, B.A.2

Source: Applied Spectroscopy, Volume 59, Issue 9, Pages 200A-220A and 1075-1187 (September 2005) , pp. 1109-1113(5)

Publisher: Society for Applied Spectroscopy

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Abstract:

Demountable calcium fluoride micro cells have been developed for use in synchrotron radiation circular dichroism (SRCD) spectroscopy. They have the advantages that they require only very small sample volumes (as low as 1 microliter) and have short path lengths (as small as 2 micrometers). Highly reproducible spectra can be obtained in consecutive loadings with these spacer-free, fixed path length cells, which enable low wavelength data collection.

Keywords: SYNCHROTRON RADIATION CIRCULAR DICHROISM SPECTROSC; SRCD; OPTICAL CELLS; ABSORPTION; STRUCTURAL GENOMICS; PROTEIN SECONDARY STRUCTURE

Document Type: Research article

DOI: 10.1366/0003702055012546

Affiliations: 1: Department of Crystallography, Birkbeck College, University of London, London, UK (F.W., B.A.W.); and Centre for Protein and Membrane Structure and Dynamics, Daresbury Laboratory, Warrington, UK (B. A. W.) 2: Department of Crystallography, Birkbeck College, University of London, London, UK (F.W., B.A.W.); and Centre for Protein and Membrane Structure and Dynamics, Daresbury Laboratory, Warrington, UK (B.A.W.)

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