Spectrofluorimetry and Chemometrics for Investigation of Norfloxacin Distribution in Multilamellar Liposomes

Authors: Zalloum, N.L.¹; Biloti, D.N.¹; Pessine, F.B.T.¹

Source: Applied Spectroscopy, Volume 59, Issue 8, Pages 166A-194A and 965-1074 (August 2005) , pp. 1032-1036(5)

Publisher: Society for Applied Spectroscopy

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Abstract:

Norfloxacin (NFX), a fluoroquinolone, was encapsulated in multilamellar liposomes (MLV) of soy-bean phosphatidylcholine at pH 7.0. The observed affinity of this class of drugs for hydrophobic environments, such as phospholipid bilayers, could lead to a better understanding of the mechanism of uptake in bacteria. The fluorescent properties of NFX were examined both free in solution and in MLV, using anisotropy and fluorescence quenching measurements. The latter data was treated with a chemometric method to deconvolute the overlapped spectra of zwitterionic and neutral species of NFX in equilibrium at this pH. The results show that NFX incorporates into the lipidic bilayers with two different distributions of species: the zwitterionic form in the lipid/aqueous interface, and the neutral one, more towards the center of the bilayer.

Keywords: NORFLOXACIN; LIPOSOMES; FLUORESCENCE; CHEMOMETRICS

Document Type: Research article

DOI: 10.1366/0003702054615179

Affiliations: 1: Instituto de Química, UNICAMP, C.P. 6154, 13084-971 Campinas, S.P., Brazil

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