Fourier Transform Infrared Imaging of Human Hair with a High Spatial Resolution Without the Use of a Synchrotron

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Abstract:

The cross-section of a human hair has been imaged for the first time using the micro attenuated total reflection (ATR) Fourier transformed infrared (FT-IR) method in combination with a focal plane array (FPA) detector. A rigorous approach was applied to determine the spatial resolution, namely, measuring the distance over which the band absorbance changes from 95 to 5% of the maximum absorbance when passing through a sharp interface. The measured value for IR transmission was ~16 μm, while the value obtained using ATR imaging was ~5 μm. The enhanced spatial resolution achieved by this method allows the medulla of the hair (~8 μm in diameter) to be imaged clearly without the need for a synchrotron source. The spatial resolution of transmission and ATR imaging is compared, and advantages of ATR imaging are discussed.

Keywords: ATR-IR; ATTENUATED TOTAL REFLECTION INFRARED; HUMAN HAIR; INFRARED SPECTROSCOPY; SPATIAL RESOLUTION

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702053085070

Affiliations: Department of Chemical Engineering and Chemical Technology, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom

Publication date: February 1, 2005

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