Accuracy and Precision of Manual Baseline Determination

Authors: Jirasek, A.1; Schulze, G.2; Yu, M.M.L.3; Blades, M.W.3; Turner, R.F.B.4

Source: Applied Spectroscopy, Volume 58, Issue 12, Pages 349A-362A and 1377-1508 (December 2004) , pp. 1488-1499(12)

Publisher: Society for Applied Spectroscopy

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Abstract:

Vibrational spectra often require baseline removal before further data analysis can be performed. Manual (i.e., user) baseline determination and removal is a common technique used to perform this operation. Currently, little data exists that details the accuracy and precision that can be expected with manual baseline removal techniques. This study addresses this current lack of data. One hundred spectra of varying signal-to-noise ratio (SNR), signal-to-baseline ratio (SBR), baseline slope, and spectral congestion were constructed and baselines were subtracted by 16 volunteers who were categorized as being either experienced or inexperienced in baseline determination. In total, 285 baseline determinations were performed. The general level of accuracy and precision that can be expected for manually determined baselines from spectra of varying SNR, SBR, baseline slope, and spectral congestion is established. Furthermore, the effects of user experience on the accuracy and precision of baseline determination is estimated. The interactions between the above factors in affecting the accuracy and precision of baseline determination is highlighted. Where possible, the functional relationships between accuracy, precision, and the given spectral characteristic are detailed. The results provide users of manual baseline determination useful guidelines in establishing limits of accuracy and precision when performing manual baseline determination, as well as highlighting conditions that confound the accuracy and precision of manual baseline determination.

Keywords: BASELINE CORRECTION; BASELINE DETERMINATION; BASELINE REMOVAL; MANUAL; SPECTRAL BASELINES

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702042641236

Affiliations: 1: Biotechnology Laboratory; Dept. of Chemistry, The University of British Columbia, Vancouver, Canada 2: Biotechnology Laboratory, The University of British Columbia, Vancouver, Canada 3: Dept. of Chemistry, The University of British Columbia, Vancouver, Canada 4: Dept. of Electrical and Computer Engineering, The University of British Columbia, Vancouver, Canada& Biotechnology Laboratory, The University of British Columbia, Vancouver, Canada

Publication date: December 1, 2004

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