Micro-Raman Spectroscopy for Optical Pathology of Oral Squamous Cell Carcinoma

Authors: Krishna, C. Murali1; Sockalingum, G.D.2; Kurien, Jacob3; Rao, Lakshmi4; Venteo, L.5; Pluot, M.5; Manfait, M.2; Kartha, V.B.6

Source: Applied Spectroscopy, Volume 58, Issue 9, Pages 254A-280A and 1023-1140 (September 2004) , pp. 1128-1135(8)

Publisher: Society for Applied Spectroscopy

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Abstract:

Micro-Raman spectra of formalin-fixed oral squamous normal and carcinoma tissues, stored at room temperature for 2 months, have been recorded. Spectra were recorded both in the epithelial and subepithelial regions of the tissues. No noticeable spectral contamination due to formalin was observed. Very significant differences between spectra of normal epithelial and malignant epithelial samples were found. No such differences in spectra of subepithelial malignant and subepithelial normal samples could be observed. This study shows that spectra from the epithelial region changes drastically because of malignancy-induced biochemical changes in this region. Major differences between normal and malignant spectra seem to arise from the protein composition, conformational/structural changes, and possible increase in protein content in malignant epithelia. The differences between normal epithelial and subepithelial spectra, as expected, arise mainly from the collagen in subepithelial tissue. Principal component analysis of the combined sets of spectra—epithelial and subepithelial, normal and malignant— showed that very good discrimination can be achieved by Raman microspectroscopy. This study thus validates the suitability of formalin-fixed tissues for optical pathology in oral malignancy.

Keywords: FORMALIN-FIXED TISSUES; MICRO-RAMAN SPECTROSCOPY; OPTICAL; ORAL CANCER; PATHOLOGY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702041959460

Affiliations: 1: Center for Laser Spectroscopy, Manipal Academy of Higher Education, Manipal-576104, India and Unité MéDIAN, CNRS UMR 6142, UFR Pharmacie, Université de Reims Champgane-Ardenne, Reims, France 2: Unité MéDIAN, CNRS UMR 6142, UFR Pharmacie, Université de Reims Champgane-Ardenne, Reims, France 3: Department of Surgical Oncology, Manipal Academy of Higher Education, Manipal-576104, India 4: Department of Pathology, Manipal Academy of Higher Education, Manipal-576104, India 5: Laboratoire Central d'Anatomie et Cytologie Pathologiques, CHU de Reims, Hôpital Robert Debré France 6: Center for Laser Spectroscopy, Manipal Academy of Higher Education, Manipal-576104, India

Publication date: September 1, 2004

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