Authors: Zhang, Dongmao; Mrozek, Melissa F.; Xie, Yong; Ben-Amotz, Dor

Source: Applied Spectroscopy, Volume 58, Issue 8, Pages 220A-244A and 887-1022 (August 2004) , pp. 929-933(5)

Publisher: Society for Applied Spectroscopy

Abstract:

A new application of the recently described drop coating deposition Raman (DCDR) method facilitates the segregation and independent spectral characterization of mixture components. The quality of the normal (un-enhanced) Raman spectra are significantly improved as a result of reduced spectral interference from fluorescent impurities and buffer compounds. Fluorescence of commercial amino acid (O-phospho-L-serine) and protein (myoglobin) samples is reduced by over an order of magnitude using DCDR, more effectively than prolonged photo-bleaching. Furthermore, DCDR is used to obtain high-quality Raman spectra of proteins, lysozyme, and insulin, derived from solutions with up to 1000-fold excess buffer concentration. Possible thermodynamic and kinetic contributions to the observed segregation phenomena are discussed.

Keywords: RAMAN; PROTEIN; PEPTIDE; AMINO ACID; BUFFER; FLUORESCENCE; SEGREGATION; PURIFICATION

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702041655430

Affiliations: 1: Purdue University, Department of Chemistry, West Lafayette, Indiana 47907

Publication date: 2004-08-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Zhang, Dongmao ;  Mrozek, Melissa F. ;  Xie, Yong ;  Ben-Amotz, Dor

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers