Glucose Quantification in Dried-Down Nanoliter Samples Using Mid-infrared Attenuated Total Reflection Spectroscopy

Authors: Diessel, Edgar; Willmann, Stefan; Kamphaus, Peter; Kurte, Roland; Damm, Uwe; Heise, H. Michael

Source: Applied Spectroscopy, Volume 58, Issue 4, Pages 98A-108A and 355-490 (April 2004) , pp. 442-450(9)

Publisher: Society for Applied Spectroscopy

Buy & download fulltext article:

OR

Price: $29.00 plus tax (Refund Policy)

Abstract:

The aim of this study was to determine the feasibility of minimally invasive glucose concentration measurement of a body fluid within the physiologically important range below 100 nL with a number of samples such as interstitial fluid, plasma, or whole blood using mid-infrared spectroscopy, but starting with preliminary measurements on samples of simple aqueous glucose solutions. The Fourier transform infrared spectrometer was equipped with a Golden Gate® single reflection diamond attenuated total reflection (ATR) accessory and a room-temperature pyroelectric detector. As the necessary detection limits can be achieved only for dried samples within the spectrometric conditions realized by a commercial instrument, the work focused on the optimization of such ATR measurements. We achieved quantification of samples with volumes as low as 7 nL between 10 and 600 mg/dL. The standard error of prediction (SEP) for the concentration range 10-100 mg/dL is 3.2 mg/dL with full interval data between 1180 and 940 cm-1. The performance of the prediction is given by a coefficient of variation of prediction (CVpred ) of 6.2%. When all samples within the whole concentration range are included, the SEP increases to 20.2 mg/dL, and hence the CVpred to 10.6% due to a nonlinear signal dependence on glucose concentration. A detection limit for glucose of 0.7 ng with a signal-to-noise ratio of 10 was obtained.

Keywords: ATR; ATTENUATED TOTAL REFLECTION; DRY FILM SAMPLE PREPARATION; GLUCOSE; INFRARED SPECTROSCOPY; MICRO-DISPENSER

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370204773580293

Affiliations: Bayer Technology Services GmbH, Competence Center Biophysics, D-51368 Leverkusen, Germany

Publication date: April 1, 2004

More about this publication?
Related content

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page