Authors: Thygesen, Lisbeth Garbrecht; Jørgensen, Kirsten; Møller, Birger Lindberg; Engelsen, Søren Balling

Source: Applied Spectroscopy, Volume 58, Issue 2, Pages 46A-64A and 143-256 (February 2004) , pp. 212-217(6)

Publisher: Society for Applied Spectroscopy

Abstract:

Cyanogenic glucosides were studied using Raman spectroscopy. Spectra of the crystal forms of linamarin, linustatin, neolinustatin, amygdalin, sambunigrin, and dhurrin were obtained using a Raman spectrograph microscope equipped with a 532 nm laser. The position of the signal from the C≡N triple bond of the cyanohydrin group was influenced by the nature of the side group and was above 2240 cm^-1 for the three cyanogenic glucosides that contain a neighboring aromatic ring, and below or partially below 2240 cm^-1 for the non-aromatic cyanoglucosides. Signals from the CN bond of linamarin/lotaustralin in leaves and roots from a medium cyanogenic cassava variety were obtained in situ using a Fourier transform near-infrared (FT-NIR) Raman interferometer with a 1064 nm laser, but the signal was very weak and difficult to obtain. A spectrum containing a signal from the CN bond of dhurrin in a freeze-dried sorghum leaf was also obtained using this instrument. Surface-enhanced Raman Spectroscopy (SERS) was demonstrated to be a more sensitive method that enabled determination of the cyanogenic potential of plant tissue. The SERS method was optimized by flow injection (FI) using a colloidal gold dispersion as effluent. Potential problems and pitfalls of the method are discussed.

Keywords: CYANOGENIC GLYCOSIDES; SURFACE-ENHANCED RAMAN SPECTROSCOPY; SERS; FLOW INJECTION; FI; CYANOHYDRINS; CN TRIPLE BOND; NITRILE BOND; CYANIDE; COLLOIDAL GOLD; CASSAVA; MANIHOT ESCULENTA; SORGHUM; SORGHUM BICOLOR; LINAMARIN; LINUSTATIN; NEOLINUSTATIN; AMYGDALIN; SAMBUNIGRIN; DHURRIN

Document Type: Research article

DOI: http://dx.doi.org/10.1366/000370204322842959

Affiliations: 1: Centre for Advanced Food Studies, Department of Dairy and Food Science, Food Technology, The Royal Veterinary and Agricultural University, 30 Rolighedsvej, DK-1958 Frederiksberg C, Denmark

Publication date: 2004-02-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Thygesen, Lisbeth Garbrecht ;  Jørgensen, Kirsten ;  Møller, Birger Lindberg ;  Engelsen, Søren Balling

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers