IngentaConnect Simultaneous Monitoring of Organic Acids and Sugars in Fresh and ...

Authors: Irudayaraj, Joseph; Tewari, Jagdish

Source: Applied Spectroscopy, Volume 57, Issue 12, Pages 349A-364A and 1455-1612 (December 2003) , pp. 1599-1604(6)

Publisher: Society for Applied Spectroscopy

Abstract:

A combination of Fourier transform infrared spectroscopy (FT-IR) and chemometrics was used as a screening tool for the determination of sugars and organic acids such as sucrose, glucose, fructose, sorbitol, citric acid, and malic acid in processed commercial and extracted fresh apple juices. Prepared samples of synthetic apple juice in different constituent concentration ranges were scanned by attenuated total reflectance (ATR) accessory and the spectral region in the range between 950 and 1500 cm^-1 was selected for calibration model development using partial least squares (PLS) regression and principal component regression (PCR). The calibration models were successfully validated by high-performance liquid chromatography (HPLC) measurements against several commercial juice varieties as well as juice extracted from different apple varieties to provide an overall R² correlation of 0.998. The present study demonstrates that Fourier transform infrared spectroscopy could be used for rapid and nondestructive determination of multiple constituents in commercial and fresh apple juices. Results indicate this approach to be a rapid and cost-effective tool for routine monitoring of multiple constituents in a fruit juice production facility.

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Simultaneous Monitoring of Organic Acids and Sugars in Fresh and Processed Apple Juice by Fourier Transform Infrared-Attenuated Total Reflection Spectroscopy