Two-Photon-Excited Phase-Resolved Fluorescence Spectroscopy
Abstract:We have constructed a new two-photon phase-resolved fluorimeter that can be used to selectively record analyte emission spectra from optically challenging samples (soft drinks, juices, and urine) without sample pretreatment. We demonstrate the use of two-photon-excited phase-resolved fluorescence (TPEPRF) by minimizing hyper-Rayleigh scatter and autofluorescence in real samples. When compared to steady-state two-photon-excited fluorescence, TPEPRF offers an improvement in signal-to-background of one to two orders of magnitude.
Document Type: Miscellaneous
Publication date: December 1, 2002
More about this publication?
- The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Request copyrighted SAS materials
- Spectroscopic Nomenclature
- Focal Point (Open Access)
- ingentaconnect is not responsible for the content or availability of external websites