If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email firstname.lastname@example.org
A method is described for the construction of three-dimensional infrared images from serial two-dimensional infrared images of various chemical structure parameters acquired from cortical bone. A series
of serial cortical bone sections, each 4-5 μm thick, separated by ~100 μm, were microtomed from PMMA-embedded normal human tibia. Twenty-three IR image cubes were acquired from this series, corresponding
to an overall sample length of ~2.2 mm. From each image cube, IR images of the spatial distribution of the mineral-to-matrix (protein) ratio, the mineral crystallinity, and the ratio of nonreducible-to-reducible
collagen crosslinks were calculated. Two-dimensional images were stacked to form a 3D view of the cortical bone, with contrast provided by variation in the spatial distribution of any of the aforementioned
chemical/molecular structural parameters.
The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)