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Direct Identification and Quantitative Determination of Acidic and Neutral Diterpenes Using 13C-NMR Spectroscopy. Application to the Analysis of Oleoresin of Pinus nigra

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We report a methodology that allows identification and quantitative determination of individual acidic and neutral diterpenes in natural mixtures, using the computer-aided analysis of their 13C-NMR spectra. The analytical procedure was validated on artificial mixtures and then applied to authentic oleoresins of Pinus nigra. Up to eleven diterpenes, which represented 63% of the total composition of the oleoresin, were identified and quantified without previous separation or derivatization.
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