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Direct Identification and Quantitative Determination of Acidic and Neutral Diterpenes Using 13C-NMR Spectroscopy. Application to the Analysis of Oleoresin of Pinus nigra

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Abstract:

We report a methodology that allows identification and quantitative determination of individual acidic and neutral diterpenes in natural mixtures, using the computer-aided analysis of their 13C-NMR spectra. The analytical procedure was validated on artificial mixtures and then applied to authentic oleoresins of Pinus nigra. Up to eleven diterpenes, which represented 63% of the total composition of the oleoresin, were identified and quantified without previous separation or derivatization.

Keywords: 13C-NMR SPECTROSCOPY; DITERPENE MIXTURE; OLEORESIN; PINUS NIGRA; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702021954890

Publication date: March 1, 2002

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