IngentaConnect Automated On-Line Noninvasive Optical Glucose Monitoring in a Cel...

Authors: Jung, Byungjo; Lee, Seungjun; Yang, In Hong; Good, Theresa; Coté, Gerard L.

Source: Applied Spectroscopy, Volume 56, Issue 1, Pages 9A-36A and 1-138 (January 2002) , pp. 51-57(7)

Publisher: Society for Applied Spectroscopy

Abstract:

Current techniques for monitoring glucose concentration during cell culture are invasively performed using an off-line measurement system. Even though in situ or ex situ analyte sensors have been tried for on-line measurement, they suffer from difficulties including the inability to keep them sterile, their limited lifetime, and their lack of stability. In this research an alternative optical noninvasive on-line monitoring system based on near-infrared absorbance spectroscopy was developed to measure glucose concentration of cell culture media in a rotary cell culture system. The system included an automatic sampling component controlled by a LabVIEW program and a high-throughput fiber coupling component connected to a Fourier transform near-infrared spectrometer. The effectiveness of the system was investigated via noninvasive on-line measurement of glucose absorbance spectra in the cell culture media during T-cell culture. Glucose absorbance spectra were collected in the spectral range of 2.0 to 2.5 μm. Partial least-squares regression was employed to build a successful multivariate calibration model. The standard error of prediction and mean percent error for glucose were 7.7 mg/dL and 1.0%, respectively. The successful results represent an important step in the development of a noninvasive, closed-loop, system for monitoring analytes in cell culture.

The full text article is available for purchase

$20.00 plus tax

Automated On-Line Noninvasive Optical Glucose Monitoring in a Cell Culture System