Method for Measuring Cellular Optical Absorption and Scattering Evaluated Using Dilute Cell Suspension Phantoms

Authors: Sefkow, A.; Bree, M.; Mycek, M.A.

Source: Applied Spectroscopy, Volume 55, Issue 11, Pages 360A-370A and 1435-1572 (November 2001) , pp. 1495-1501(7)

Publisher: Society for Applied Spectroscopy

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Abstract:

The opal glass method (OGM) is a standard technique used by the ocean optics community to measure the spectral absorption coefficients of algal suspensions. We present and evaluate this method for its potential application to measurements of the inherent optical properties (optical absorption and scattering coefficients) of dilute mammalian cell suspensions, as an alternative to the integrating sphere method. In a series of controlled experiments on absorbing and scattering phantoms (Evans blue dye and polystyrene spheres 1-20 μm in diameter), separation of absorption and scattering was possible with less than 20% error in absorption coefficient estimates for particles with major scattering components in the size range of 2-5 μm.

Keywords: OPTICAL SPECTROSCOPY; BIOMEDICAL OPTICS; SCATTERING; BIOLOGICAL PHANTOMS

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702011953711

Publication date: 2001-11-01

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