If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Method for Measuring Cellular Optical Absorption and Scattering Evaluated Using Dilute Cell Suspension Phantoms

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The opal glass method (OGM) is a standard technique used by the ocean optics community to measure the spectral absorption coefficients of algal suspensions. We present and evaluate this method for its potential application to measurements of the inherent optical properties (optical absorption and scattering coefficients) of dilute mammalian cell suspensions, as an alternative to the integrating sphere method. In a series of controlled experiments on absorbing and scattering phantoms (Evans blue dye and polystyrene spheres 1-20 μm in diameter), separation of absorption and scattering was possible with less than 20% error in absorption coefficient estimates for particles with major scattering components in the size range of 2-5 μm.

Keywords: BIOLOGICAL PHANTOMS; BIOMEDICAL OPTICS; OPTICAL SPECTROSCOPY; SCATTERING

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702011953711

Publication date: November 1, 2001

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more