Authors: Reis, Marlon M.; Biloti, Débora N.; Ferreira, Márcia M.C.; Pessine, Francisco B.T.; Teixeira, Gustavo M.

Source: Applied Spectroscopy, Volume 55, Issue 7, Pages 225A-248A and 809-951 (July 2001) , pp. 847-851(5)

Publisher: Society for Applied Spectroscopy

Abstract:

Chromophore identification in biological samples often requires the physical separation of the compounds, which can be difficult. Although there are several advantages to hyphenated spectroscopic techniques for identification of substances, complex mixtures of chromophores presenting overlapped spectra cannot be identified directly through this method. This work presents an application of chemometrics to compound identification in biological samples by a spectroscopic hyphenated technique using a curve resolution method. The PARAllel FACtor analysis model (PARAFAC), which has no rotational indeterminacy, was used for curve resolution of excitation-emission spectra of human dental tartars. PARAFAC was applied under constraints (i.e., unimodality and non-negativity) and evaluated with a validation procedure. The resolved profiles are porphyrinic-like spectra presenting excitation band maxima at 407, 416, and 431 nm in the Soret band region (390-440 nm) of these substances.

Keywords: PORPHYRINIC EXCITATION-EMISSION SPECTRA; PARAFAC; CURVE RESOLUTION; TARTAR

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702011952875

Publication date: 2001-07-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Reis, Marlon M. ;  Biloti, Débora N. ;  Ferreira, Márcia M.C. ;  Pessine, Francisco B.T. ;  Teixeira, Gustavo M.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers