If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Simultaneous Fluorimetric Determination of Pteridin Derivatives: Comparison between Synchronous, Partial Least-Squares, and Hybrid Linear Analysis Methods

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Several methods are described for the simultaneous determination of binary mixtures of xanthopterin (XAN) and isoxanthopterin (ISO) and of binary mixtures of xanthopterin and biopterin (BIO). The total luminiscence information from each compound has been used to optimize the most adequate analytical signal. A synchronous spectrofluorometric univariate procedure to analyze XAN and ISO is proposed. The procedure is compared with two multivariate procedures based on partial least-squares (PLS-1) and hybrid linear analysis (HLA/XS), by using synchronous spectral data as analytical signals. For the resolution of binary mixtures of XAN and BIO, a comparison between the predictive ability of the multivariate calibration methods, PLS-1 and HLA/XS, on two spectral data sets, excitation and emission spectra, has been performed. The calculation of the net analyte signal (NAS) by the HLA/XS method allows the determination of selectivity and sensitivity in each case. The proposed procedures are validated in the determination of several mixtures of these compounds, and the error values in a test set are reported.

Keywords: HLA; HYBRID LINEAR ANALYSIS; PARTIAL LEAST-SQUARES; PTERIDINS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702011952622

Publication date: June 1, 2001

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more