If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Multivariate Calibration for the Determination of Analytes in Urine Using Mid-Infrared Attenuated Total Reflection Spectroscopy

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The reagent-free multicomponent analysis of components in urine using mid-infrared spectroscopy possesses many attractions. A population of 67 individual urine samples from children and adults, collected over 24 h, was analyzed for urea, creatinine, uric acid, glucose, total protein, phosphate, and sulfate by using clinical reference methodology. The urine pH value was potentiometrically measured by a glass electrode. Partial least-squares (PLS) calibration models were calculated over optimized, component-specific ranges from attenuated total reflection spectra of the urine samples measured by a micro-Circle cell. Apart from glucose and total protein, for which the spread in urine sample concentrations was too small, calibrations were successful for metabolites such as urea, creatinine, and uric acid. Additionally, concentrations of sulfate and phosphate anions, which show significant mid-infrared absorption bands, could also be quantified. The acid secreted with the urine influences the equilibrium between di- and monobasic phosphate in this biofluid, which is used as the spectroscopic basis for the pH assay presented here. The analytical performance of the reference methods is discussed with regard to evaluating the limitations of the spectroscopic assay. Additionally, aqueous solutions of individual urine components with a spread of concentrations similar to those found in native urine samples were analyzed by using PLS calibrations.

Keywords: ATR; COMPONENTS IN URINE; MID-INFRARED ATTENUATED TOTAL REFLECTION SPECTROSCOPY; MULTIVARIATE CALIBRATION; PARTIAL LEAST-SQUARES; PLS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702011951948

Affiliations: 1: Institut für Spektrochemie und Angewandte Spektroskopie, Bunsen-Kirchhoff-Str. 11, D-44139 Dortmund, Germany 2: Institut für Ernährungswissenschaft, Justus-Liebig-Universität Giessen, Wilhelmstraße 20, D-35392 Giessen, Germany 3: Roche Diagnostics, D-68305 Mannheim, Germany

Publication date: April 1, 2001

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more