Development of a Raman Detector for Capillary Electrophoresis

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Abstract:

Efficient detector optics incorporating a hyperhemispherical element have been developed to facilitate the coupling of capillary electrophoresis or microchromatography with Raman spectroscopy. The design has a high tolerance for alignment and focusing errors. A simple model has been developed to predict the behavior of the system as capillary diameter is varied. This approach was found to provide good qualitative agreement with experiment. Limits of detection in capillary electrophoresis experiments without preconcentration were found to be ~ 5 mM for nitrate and perchlorate ions. Direct detection of several carbohydrates was also demonstrated; with the use of sample stacking, limits of detection were in the millimolar range. The analytes were distinguishable by their Raman spectra.

Keywords: CAPILLARY; CARBOHYDRATES; DESIGN; DETECTOR; ELECTROPHORESIS; RAMAN; SPECTROSCOPY

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702001948970

Affiliations: 1: Department of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, U.K.; 2: School of Chemistry, University of Leeds, Leeds, LS2 9JT, U.K.; 3: Waters Corp., 34 Maple St., Milford, Massachusetts 01757-3696, U.S.A. 4: Renishaw plc, Old Town, Wotton-under-Edge, Glos, GL12 7DH, U.K.

Publication date: December 1, 2000

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