Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.

Keywords: CONFOCAL; FLUORESCENCE; IMAGING; MICROSCOPY; SPECTRAL

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702001948989

Affiliations: 1: Department of Chemistry, Duke University, Durham, North Carolina 27708 2: Department of Chemistry, Duke University, Durham, North Carolina 27708 and Department of Biochemistry, Duke University Medical Center, Duke University, Durham, North Carolina 27710

Publication date: December 1, 2000

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more