Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy

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This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.


Document Type: Research Article


Affiliations: 1: Department of Chemistry, Duke University, Durham, North Carolina 27708 2: Department of Chemistry, Duke University, Durham, North Carolina 27708 and Department of Biochemistry, Duke University Medical Center, Duke University, Durham, North Carolina 27710

Publication date: December 1, 2000

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