Light-Induced Two-Dimensional FT-IR Spectroscopy of BacterioRhodopsin

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Two-dimensional Fourier transform infrared (2D FT-IR) spectroscopy was applied to study the slower states of the membrane protein bacterioRhodopsin (bR) photocycle, with bR adsorbed on a ZnSe attenuated total reflectance (ATR) crystal. The M and the N states of the bR photocycle could be discriminated, and 2D spectra revealed an N state absorption band at 1536 cm-1 that is normally covered by the broader and bigger 1527 cm-1 M state absorption band. 2D FT-IR absorbance changes are presented in a more convenient way for analysis than in traditional 2D FT-IR spectroscopy by using 2D-phase spectra and phase/amplitude calculations.


Document Type: Research Article


Affiliations: Bio-Interface Group, Department of Applied Physics, Twente University, P.O. Box 217, NL 7500 AE Enschede, The Netherlands

Publication date: November 1, 2000

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