Current techniques used to monitor glucose concentration of cell culture media (CCM) require invasive and tedious handling of the sample for sterile media removal and nutrient replacement. In order to optimize
cell culture growth in bioreactors, biosensors must be developed that are capable of monitoring the cell culture processes noninvasively and continuously. In this study, on-line, noninvasive determinations
of glucose in cell culture media were investigated via near-infrared spectroscopy (NIR) across the 2.0-2.5 μm combination region. A system was developed, using a unique fiberoptic coupling method and
a commercial Fourier transform infrared (FT-IR) spectrometer, to characterize glucose single-beam spectra collected from cell culture media. This novel system is the first of its kind and integrates a completely
noninvasive optical probe to measure glucose concentrations within cell culture media, in situ. Spectra recorded from a four-day fibroblast culture with this fiber coupled system and an FT-IR spectrometer
have been analyzed and compared with standard clinical chemistry techniques. Partial least-squares (PLS) regression has been used to extract the analyte-dependent information and to build a successful multivariate
calibration model. A combination of spectra from cell culture media and prepared media mixtures was used to eliminate unwanted correlations in the calibration data. The combined use of this unique fiber-optic
system, PLS, and uncorrelated spectra resulted in a true glucose prediction error of 14.8 mg/dL in an independent validation set.
Biomedical Engineering Program, MS 3120, Texas A and M University, College Station, Texas 77843 2:
BioTex Inc, 1511 South Texas Avenue, PMB 197, College Station, Texas 77840
Publication date: October 1, 2000
More about this publication?
The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)