Solid-Matrix Fluorescence and Phosphorescence Detection and Characterization of Benzo\[a]pyrene-DNA Adducts with Whatman No. 1 and Whatman 1PS Filter Paper
Abstract:Benzo\[a]pyrene (B\[a]P) is a potential carcinogen and mutagen, and it has been studied very extensively. In humans, B\[a]P can be metabolized and becomes chemically bonded to DNA. A variety of methods are needed for the characterization of B\[a]P-DNA adducts because of the structural complexity of the adducts. In this work, several solid-matrix luminescence methods were developed for the characterization of the B\[a]P-DNA adducts. Both solid-matrix fluorescence and solid-matrix phosphorescence could be obtained from the adducts on Whatman No. 1 and Whatman 1PS filter paper at nanogram levels. Thallium nitrate proved to be an effective heavy atom in enhancing the solid-matrix phosphorescence of the adducts. By selective excitation of the phosphorescence of the adducts, it was possible to excite different forms of the adducts. With moisture quenching of the solid-matrix phosphorescence of the B\[a]P-DNA adducts, it was feasible to selectively detect quasi-intercalated adducts. With Whatman 1PS filter paper, a unique method was developed for the solid-matrix fluorescence detection of adducts externally bound to DNA by the hydrophobic interaction of the adducts with the 1PS paper.
Document Type: Research Article
Publication date: March 1, 1998
More about this publication?
- The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Request copyrighted SAS materials
- Spectroscopic Nomenclature
- Focal Point (Open Access)
- ingentaconnect is not responsible for the content or availability of external websites