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Solid-Matrix Fluorescence and Phosphorescence Detection and Characterization of Benzo\[a]pyrene-DNA Adducts with Whatman No. 1 and Whatman 1PS Filter Paper

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Abstract:

Benzo\[a]pyrene (B\[a]P) is a potential carcinogen and mutagen, and it has been studied very extensively. In humans, B\[a]P can be metabolized and becomes chemically bonded to DNA. A variety of methods are needed for the characterization of B\[a]P-DNA adducts because of the structural complexity of the adducts. In this work, several solid-matrix luminescence methods were developed for the characterization of the B\[a]P-DNA adducts. Both solid-matrix fluorescence and solid-matrix phosphorescence could be obtained from the adducts on Whatman No. 1 and Whatman 1PS filter paper at nanogram levels. Thallium nitrate proved to be an effective heavy atom in enhancing the solid-matrix phosphorescence of the adducts. By selective excitation of the phosphorescence of the adducts, it was possible to excite different forms of the adducts. With moisture quenching of the solid-matrix phosphorescence of the B\[a]P-DNA adducts, it was feasible to selectively detect quasi-intercalated adducts. With Whatman 1PS filter paper, a unique method was developed for the solid-matrix fluorescence detection of adducts externally bound to DNA by the hydrophobic interaction of the adducts with the 1PS paper.

Keywords: PYRENE-DNA ADDUCTS ROOM-TEMPERATURE FLUORESCENCE ROOM-TEMPERATURE PHOSPHORESCENCE

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702981943608

Publication date: March 1, 1998

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