Use of Transmitted Room-Temperature Phosphorescence to Improve Nalidixic Acid Determination

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

A method for nalidixic acid (NA) determination by transmitted room-temperature phosphorescence is proposed. The antibacterial agent NA in acidic solution shows native fluorescence; when NA is spotted on a filter paper precoated with beta -cyclodextrin and lead (II) acetate and dried for 5 min, phosphorescence results. The transmitted phosphorescence was measured directly with the use of a homemade sample holder. The excitation and emission wavelengths were 330 and 417 nm, respectively. The applicable concentration range was between 0.06 and 4.50 mu g/mL with a relative standard deviation of 2.8% and a detection limit of 0.02 mu g/mL. The method was applied to the determination of NA in human urine and pharmaceuticals, with recoveries from 97.5 to 105.5%.

Keywords: PHOSPORESCENCE NALIDIXIC ACID PHARMACEUTICAL ANALYSIS BIOLOGICAL FLUID ANALYSIS

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702981942393

Publication date: January 1, 1998

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more