The instrumentation development of a fluorescence detector for flow injection analysis (FIA) based on an acousto-optic tunable filter (AOTF) is reported. The unique features of the AOTF-namely, its all-solid-state, compact, fast-scanning, and high-resolution characteristics-make it
possible to develop the first fluorescence detector for FIA which is capable of recording the whole fluorescence spectrum with fine vibronic structure of the compound as it is flowing through the FIA detector. This capability, in turn, makes it possible to use this detector for the determination
of the polarity of any sample by doping it with a small amount of pyrene and measuring the ratio of fluorescence intensities of band 1 and band 3. Because the sample in this case is flowing through the FIA instrument, it can be a mixture of two different solvents or surfactants by operating
the FIA instrument under gradient condition. Polarity of any mixtures of solvents and surfactants can be simply, rapidly, and economically (in terms of the amount of samples and the time needed for measurements) determined.
Department of Chemistry, Marquette University, P.O. Box 1881, Milwaukee, Wisconsin 53201
Publication date: December 1, 1996
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