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Applications of a New 206.5-nm Continuous-Wave Laser Source: UV Raman Determination of Protein Secondary Structure and CVD Diamond Material Properties

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Abstract:

We demonstrate the utility of a new 206.5-nm continuous-wave UV laser excitation source for spectroscopic studies of proteins and CVD diamond. Excitation at 206.5 nm is obtained by intracavity frequency doubling the 413-nm line of a krypton-ion laser. We use this excitation to excite resonance Raman spectra within the π → π amide transition of the protein peptide backbone. The 206.5-nm excitation resonance enhances the protein amide vibrational modes. We use these high signal-to-noise spectral data to determine protein secondary structure. We also demonstrate the utility of this source to excite CVD and gem-quality diamond within its electronic band-gap. The diamond Raman spectra have very high signal-to-noise ratios and show no interfering broad-band luminescence. Excitation within the diamond bandgap also gives rise to narrow photoluminescence peaks from diamond defects. These features have previously been observed only by cathodoluminescence measurements. This new continuous-wave UV source is superior to the previous pulsed sources, because it avoids nonlinear optical phenomena and thermal sample damage; Photoluminescence.

Keywords: CVD diamond; Instrumentation; Lasers; Photoluminescence; Protein secondary structure studies; Raman spectroscopy; UV resonance Raman spectroscopy

Document Type: Rapid Communication

DOI: http://dx.doi.org/10.1366/0003702963904683

Affiliations: 1: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 2: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260; present address: Department of Chemistry, University of California-Los Angeles, Los Angeles, CA 90024 3: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260; present address: Section on Metabolic Analysis and Mass Spectrometry, NICHD, National Institute of Health, 10 Center Drive, MSC1580, Room 6C208, Bethesda, MD 20892 4: Coherent Laser Group, Division of Coherent Inc., 5100 Patrick Henry Drive, Santa Clara, California 95056

Publication date: November 1, 1996

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