Effects of a Heavy-Atom Salt on the Solid-Matrix Luminescence Properties of the (±)-trans-7,8-Dihydroxy-anti-9,10-Epoxy-7,8,9,10-Tetrahydrobenzo[a]pyrene-DNA Adducts and Tetrol I-1 Adsorbed on Several Solid Matrices

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Abstract:

The effects of a heavy-atom salt on the room-temperature solid-matrix luminescence properties of the (±)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts [(±)-anti-BPDE-DNA adductsl and benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (tetrol I-1) on several solid matrices were investigated. TlNO3 was found to be very effective in enhancing the room-temperature phosphorescence of the (±)-anti-BPDE-DNA adducts and tetrol I-1. However, the room-temperature fluorescence signals were quenched from the (±)-anti-BPDE-DNA adducts and tetrol I-1 on the solid matrices with the heavy-atom salt. It was found that 1% α-cyclodextrin/TlNO3, 10% trehalose/TlNO3, and 30% TlNO3/sodium acetate (NaOAc) yielded very strong RTP signals from the (±)-anti-BPDE-DNA adducts. The greatest enhancement of the RTP intensities from the (±)-anti-BPDE-DNA adducts was achieved with 30% TlNO3/NaOAc. In addition, a very low limit of detection (0.14 fmole/mg) for tetrol I-1 was acquired with 30% TlNO3/NaOAc. With its tremendous enhancement of RTP signals from the adducts and tetrol I-1 and the sub-femtomole limit of detection obtained for tetrol I-l, 30% TlNO3/NaOAc is ideal for the characterization and analysis of DNA adducts and their tetrol products.

Keywords: Benzo[a]pyrene-DNA adducts; Cyclodextrin; Heavy-atom effect; Sodium acetate; Solid-matrix luminescence; Tetrols; Trehalose

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702963906159

Affiliations: Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071

Publication date: April 1, 1996

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