Effects of a Heavy-Atom Salt on the Solid-Matrix Luminescence Properties of the (±)-trans-7,8-Dihydroxy-anti-9,10-Epoxy-7,8,9,10-Tetrahydrobenzo[a]pyrene-DNA Adducts and Tetrol I-1 Adsorbed on Several Solid Matrices

$29.00 plus tax (Refund Policy)

Buy Article:


The effects of a heavy-atom salt on the room-temperature solid-matrix luminescence properties of the (±)-trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-DNA adducts [(±)-anti-BPDE-DNA adductsl and benzo[a]pyrene-r-7,t-8,9,c-10-tetrahydrotetrol (tetrol I-1) on several solid matrices were investigated. TlNO3 was found to be very effective in enhancing the room-temperature phosphorescence of the (±)-anti-BPDE-DNA adducts and tetrol I-1. However, the room-temperature fluorescence signals were quenched from the (±)-anti-BPDE-DNA adducts and tetrol I-1 on the solid matrices with the heavy-atom salt. It was found that 1% α-cyclodextrin/TlNO3, 10% trehalose/TlNO3, and 30% TlNO3/sodium acetate (NaOAc) yielded very strong RTP signals from the (±)-anti-BPDE-DNA adducts. The greatest enhancement of the RTP intensities from the (±)-anti-BPDE-DNA adducts was achieved with 30% TlNO3/NaOAc. In addition, a very low limit of detection (0.14 fmole/mg) for tetrol I-1 was acquired with 30% TlNO3/NaOAc. With its tremendous enhancement of RTP signals from the adducts and tetrol I-1 and the sub-femtomole limit of detection obtained for tetrol I-l, 30% TlNO3/NaOAc is ideal for the characterization and analysis of DNA adducts and their tetrol products.

Keywords: Benzo[a]pyrene-DNA adducts; Cyclodextrin; Heavy-atom effect; Sodium acetate; Solid-matrix luminescence; Tetrols; Trehalose

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702963906159

Affiliations: Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071

Publication date: April 1, 1996

More about this publication?
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more