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High-Fidelity Fourier Transform Infrared Spectroscopic Imaging of Primate Brain Tissue

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Abstract:

We demonstrate a new mid-infrared and near-infrared imaging approach which is ideally suited to microscopic applications. The method employs an indium antimonide (InSb) focal-plane array detector and a commercially available step-scan Fourier transform infrared spectrometer (FTIR). With either a KBr or a CaF2 beamsplitter, images from 1 to 5.5 μm (10,000–1818 cm-1) can be rapidly acquired with the use of all the available pixels on the detector. The spectral resolution for each image is easily varied by changing the number of acquired images during the interferometer scan. We apply this technique to noninvasively generate image contrast in sections of monkey brain tissue and to relate these data to specific lipid and protein fractions. In addition, we describe several computational methods to highlight the spatial distributions of components within a sample.

Keywords: Brain tissue; Chemical imaging; Fourier transform infrared spectroscopy; Infrared microscopy; Vibrational spectroscopic imaging

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702963906618

Affiliations: 1: Laboratory of Chemical Physics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 2: The Procter and Gamble Company, Miami Valley Laboratories, Cincinnati, Ohio 45253-8707

Publication date: February 1, 1996

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