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Characteristics of a Rubidium Metal Vapor Filter for Laser Scatter Rejection in Single Molecule Detection

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Abstract:

The characteristics of a rubidium metal vapor filter (MVF) for its use as a laser scatter filter for single molecule detection (SMD) were investigated. This investigation was done by a simulation along with experimental observation. A monochromator was used to elucidate the spectral components of the background in SMD. The major contributors to the background were laser transmission and fluorescence of the rubidium vapor. Experiments on the filter's performance under varying conditions such as MVF temperature, MVF pressure, and laser power were done. It was found that the integrated transmission intensity decreased with increasing pressure and temperature while it increased for increasing laser powers. The computer simulation modeled the experimentally observed profiles very well. Through the simulation, it was determined that the limitation on the attenuation by the MVF was due to the transmission of the broad-band stimulated emission "wings" of the Ti:sapphire laser.

Keywords: Lase-rinduced fluorescence; Metal vapor filters; Near-infrared; Single molecule detection; Spectral simulation

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702963906564

Affiliations: 1: Department of Chemistry, University of Florida, Gainesville, Florida 32611 2: Department of Chemistry, University of Florida, Gainesville, Florida 32611; present address: Oak Ridge National Laboratory, Chemical and Analytical Sciences Division, P.O. Box 2008, Oak Ridge, TN 37831 3: Department of Chemistry, University of Florida, Gainesville, Florida 32611; Institute of Spectroscopy Russian Academy of Sciences, 142092 Troitzk Moscow Region, Isan, Russia 4: Department of Chemistry, University of Florida, Gainesville, Florida 32611; present address: Oak Ridge National Laboratory, Bldg. 4500S, P.O. Box 2008, Oak Ridge, TN 37831

Publication date: February 1, 1996

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