Time-Dependent Fluorescence Depolarization Analysis in Three-Dimensional Microspectroscopy

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Abstract:

Three-dimensional space- and time-resolved fluorescence depolarization spectroscopy has been developed for elucidating picosecond rotational relaxation processes occurring in micrometer-sized volumes. Anisotropy decays are obtained by a new analytical method which is adapted to the large-solid-angle characteristic of the microscope optics. System parameters introduced in this method were determined by measuring a reference sample. The fluorescence dynamics of p-terphenyl in a thin liquid layer was analyzed as a demonstration experiment.

Keywords: Fluorescence depolarization; Microspectroscopy; Rotational relaxation; Three-dimensional resolution; Time-resolved spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702953963652

Affiliations: 1: Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Kyoto Research Park, Chudoji-minami, Shimogyo-ku, Kyoto 600, Japan; present address: Kaneka Corporation, Elmech Business Development Section, R&D Group, 5-1-1, Torigai-Nishi, Settsu, Osaka 566, Japan 2: Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Kyoto Research Park, Chudoji-minami, Shimogyo-ku, Kyoto 600, Japan; present address: Department of Applied Physics, Osaka University, Suita, Osaka 565, Japan

Publication date: February 1, 1995

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