Skip to main content

Determination of Chlorophyll a and Pheophytin a Concentrations in Acetone Extracts by Laser Fluorometry

Buy Article:

$29.00 plus tax (Refund Policy)

Abstract:

We have used the technique of laser fluorometry for chlorophyll a and pheophytin a measurements in 90% acetone/water extracts of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic of a pulsed Nd:YAG laser (λ = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process of individual zooplankton samples.

Keywords: Acetone extract; Chlorophyll; Laser fluorometer; Pheophytin; Phytoplankton; Zooplankton

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702953963715

Affiliations: Department of Physics, Moscow University, 119899 Moscow, Russia

Publication date: February 1, 1995

More about this publication?
sas/sas/1995/00000049/00000002/art00009
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more