Determination of Chlorophyll a and Pheophytin a Concentrations in Acetone Extracts by Laser Fluorometry

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

We have used the technique of laser fluorometry for chlorophyll a and pheophytin a measurements in 90% acetone/water extracts of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic of a pulsed Nd:YAG laser (λ = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process of individual zooplankton samples.

Keywords: Acetone extract; Chlorophyll; Laser fluorometer; Pheophytin; Phytoplankton; Zooplankton

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702953963715

Affiliations: Department of Physics, Moscow University, 119899 Moscow, Russia

Publication date: February 1, 1995

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more