We have used the technique of laser fluorometry for chlorophyll a and pheophytin a measurements in 90% acetone/water extracts of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic
of a pulsed Nd:YAG laser (λ = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process
of individual zooplankton samples.
Department of Physics, Moscow University, 119899 Moscow, Russia
Publication date: February 1, 1995
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