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Use of an Optical-Fiber-Based Biosensor to Study the Interaction of Blood Proteins with Solid Surfaces

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Abstract:

An optical fiber-based laser biosensor has been used to investigate adsorption of blood lipoproteins and immunoglobulin G to the glass optical-fiber surface. While lipoproteins readily interact (adsorb) with unmodified optical-fiber surface, immunoglobulin G does not bind to the glass surface at a concentration of 5 μg/mL. However, upon glass surface modification with silane, immunoglobulin G binds significantly to the optical fiber. High-density lipoproteins and low-density lipoproteins show a differential interaction with the glass surface, and their interaction was altered upon modification of the glass surface with cholesterol. With these results in mind, the utility of the biosensor for the study of protein interaction with biorelevant surfaces has been outlined.

Keywords: Adsorption; Binding kinetics; Biosensor; Blood proteins; Fluorescence signal; Lipoproteins; Optical fiber

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702944030026

Affiliations: Department of Chemistry, University of Massachusetts-Dartmouth, North Dartmouth, Massachusetts 02747

Publication date: July 1, 1994

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