Use of FT-IR for the Quantification of Water in Biomolecules
Abstract:Water (10-3 mg H2O in 1 mg of biopolymer) was extracted from biological compounds with acetonitrile and determined by FT-IR spectrometry with the use of a KBr transmission cell. A nitrogen-gas-purged glove box was employed during the extraction to avoid errors due to water absorption from ambient laboratory air. The subtractive compensation for concomitant water in acetonitrile was further accomplished by using a reference spectrum of acetonitrile kept under the same experimental conditions as those for preparing the acetonitrile extracts of water from the testing samples. This technique was applied to the quantification of water in peptides and enzymes. The advantages of this technique over the Karl Fisher titration are discussed, along with measurements for loss of weight on drying in vacuo.
Document Type: Research Article
Affiliations: Analytical and Metabolic Research Laboratories, Sankyo Co., Ltd., 1-2-58 Hiromachi, Shinagawa-ku, Tokyo 140, Japan
Publication date: July 1, 1994
More about this publication?
- The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Request copyrighted SAS materials
- Spectroscopic Nomenclature
- Focal Point (Open Access)
- ingentaconnect is not responsible for the content or availability of external websites