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Indium Antimonide (InSb) Focal Plane Array (FPA) Detection for Near-Infrared Imaging Microscopy

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Abstract:

Near-infrared spectroscopy is a sensitive, noninvasive method for chemical analyses, and its integration with imaging technologies represents a potent tool for the study of a wide range of materials. In this communication the use of an indium antimonide (InSb) multichannel imaging detector for near-infrared absorption spectroscopic microscopy is described. In particular, a 128 × 128 pixel InSb staring array camera has been combined with a refractive optical microscope and an acousto-optic tunable filter (AOTF) to display chemically discriminative, spatially resolved, vibrational spectroscopic images of biological and polymeric systems. AOTFs are computer-controlled bandpass filters that provide high speed, random wavelength access, wide spectral coverage, and high spectral resolution. Although AOTFs inherently have a wide range of spectroscopic applications, we apply this technology to NIR absorption microscopy between 1 and 2.5 μm. The spectral interval is well matched to the optical characteristics of both the NIR refractive microscope and the AOTF, thereby providing near-diffraction-limited performance with a practical spatial resolution of 1 to 2 μm. Design principles of this novel instrumentation and representative applications of the technique are presented for various model systems.

Keywords: Acousto-optic tunable filter (AOTF); Acousto-optics; Biological imaging; Chemical imaging; Focal plane array detectors; Indium antimonide; Infinity-corrected optics; Multichannel detectors; Near-infrared spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702944924899

Affiliations: 1: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 2: Laboratory of Chemical Physics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 3: Laboratory of Chemical Physics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892; address for correspondence: National Institutes of Health, Building 5, Room BI-38, Bethesda, MD 20892

Publication date: May 1, 1994

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