Micron Surface-Enhanced Raman Spectroscopy of Intact Biological Organisms and Model Systems

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Abstract:

Surface-enhanced Raman spectra have been obtained within intact zebrafish embryos and inside the 500-fL pores of a Nucleopore filter membrane with the use of coated microelectrodes with 1-3 μm active silver tip diameters. The spectra obtained demonstrate the microelectrode's ability to penetrate biological membranes as well as restricted volumes.

Keywords: Biological organism; Microelectrode; Raman spectroscopy; Surface-enhanced Raman spectroscopy

Document Type: Rapid Communication

DOI: http://dx.doi.org/10.1366/0003702944924790

Affiliations: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055

Publication date: May 1, 1994

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