Skip to main content

Measurement of Hemoglobin in Unlysed Blood by Near-Infrared Spectroscopy

Buy Article:

$29.00 plus tax (Refund Policy)


Visible and near-infrared transmittance (T) spectra of unlysed blood samples were obtained with an NIRSystems Model 6500 spectrophotometer modified for an open cell and a vertical light path. Without rigid control of the pathlength and temperature, we were able to measure hemoglobin content within a standard error of 0.43 g/dL using a single-term second-derivative ratio of log(1/T) data at 1740 and 1346 nm. Calibration was done on a set of 104 samples (two spectra of blood from 52 patients) having hemoglobin levels of 6.1 to 19.2 g/dL. Validation was done on an independent set of 56 samples (two spectra of blood from 28 patients) having hemoglobin levels of 7.2 to 19.0 g/dL. The reproducibility of the measurement, tested by computing the coefficient of variability of the 28 duplicated results, was 0.63% (<0.1 g/dL). The results demonstrate a rapid simple diffuse transmittance measurement of hemoglobin in unlysed blood.

Keywords: Concentration; Hemoglobin; Near-infrared

Document Type: Research Article


Affiliations: 1: Department of Pathology, The Valley Hospital, 223 North Van Dien Avenue, Ridgewood, New Jersey 07450 2: Consultant, 11204 Montgomery Road, Beltsville, Maryland 20705 3: Department of Biostatistics, Henry M. Jackson Foundation, 1401 Rockville Pike, Suite 600, Rockville, Maryland 20852

Publication date: April 1, 1994

More about this publication?

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more