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Confocal Raman Microspectroscopy Using a Stigmatic Spectrograph and CCD Detector

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Confocal Raman microspectroscopy has previously used pinholes placed at the back focal plane of the microscope to provide depth resolution along the optical axis. The process of optimizing the pinhole alignment can often be difficult and time-consuming. We demonstrate a different approach to setting up a confocal Raman microscope using a stigmatic spectrograph and a CCD detector. This arrangement is easy to use and provides a depth resolution of ∼2 μm.

Keywords: Confocal microscopy; Microspectroscopy; Raman spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702944028407

Affiliations: 1: Renishaw Transducer Systems Limited, Old Town, Wotton Under Edge, Gloucestershire, GL12 7DG, U.K. 2: Department of Physics, University of Leeds, Leeds, U.K. 3: DSM Research, P.O. Box 18, 6160MD Geleen, The Netherlands

Publication date: February 1, 1994

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