Skip to main content

Depth Profile Analysis of Keratin Fibers by FT-IR Photoacoustic Spectroscopy

Buy Article:

$29.00 plus tax (Refund Policy)


The depth profiling capability of Fourier transform infrared photoacoustic spectroscopy (FT-IR/PAS) has been used to distinguish spectroscopically between the surface layer (cuticle) and the interior (cortex) of intact keratin fibers. The thermal diffusion lengths of wool and hair fibers, calculated as a function of the wavenumber and optical scanning velocity of the interferometer, indicate a depth resolution of the order of a micrometer. Shifts in the amide I and II bands to lower wavenumbers were observed with increasing velocity of the interferometer, consistent with differences in protein structure between the bulk and surface regions of the fiber. On the basis of these spectral changes, the location of the cuticle/cortex interface below the fiber surface can be determined.

Keywords: Depth profiling; FT-IR/PAS; Hair; Keratin; Photoacoustic spectroscopy; Wool

Document Type: Research Article


Affiliations: 1: School of Chemistry, University of Melbourne, Parkville, Victoria 3052, Australia 2: Dulux Australia, Research and Development Laboratory, Clayton, Victoria 3168, Australia 3: Bio-Rad Laboratories, Digilab Division, North Ryde, N. S. W. 2133, Australia

Publication date: January 1, 1994

More about this publication?

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more